Question

Insulin getting assembled into a mature form was the major challenge in commercial insulin production by rDNA technology. How did Eli Nilly found a solution to this problem?

Answer 1

An American company Eli Lilly in 1983 prepared two DNA sequences corresponding to A and B, chains of human insulin and inserted in plasmids of E, coli to produce insulin chains. Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin.