Use app×
Join Bloom Tuition
One on One Online Tuition
JEE MAIN 2025 Foundation Course
NEET 2025 Foundation Course
CLASS 12 FOUNDATION COURSE
CLASS 10 FOUNDATION COURSE
CLASS 9 FOUNDATION COURSE
CLASS 8 FOUNDATION COURSE
0 votes
910 views
in Biotechnology: Principles and Processes by (48.9k points)
closed by

In order to force bacteria to take up the plasmid, the bacterial cells must first be made ‘competent’ to take up DNA. Suggest a method to make bacterial cell competent to receive DNA.

1 Answer

+1 vote
by (58.8k points)
selected by
 
Best answer

At first, treat the cell with divalent cation like calcium, which increases the efficiency with which DNA enters the bacterium through pores in the cell wall. Then incubate of cell with rDNA on ice and placing them briefly at 42° C and then putting back to ice.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.

...