Question

Stanley cohen and Hebert Boyer constructed r DNA by using plasmid and antibiotic resistant gene.

1. Name the slicing and splicing enzyme used.

2. What is the significance of slicing enzyme in rDNA technology?

Answer 1

1. Slicing enzyme – Restriction endo nuclease Splicing enzyme-DNA ligase

2. significance of slicing enzyme in rDNA technology: 

• It is used to cut within DNA sequence.
• Same restriction enzyme is used to cut both vector DNA and foreign DNA.