Question

Following are the various steps of recombinant DNA technology.

Arrange them in correct sequential order.

(i) Obtaining the foreign gene product

(ii) Amplication of gene of interest using PCR

(iii) Cutting of DNA at specific locations 

(iv) Downstream processing 

(v) Intsertion of recombinant DNA into the host cell/organism

(vi) Isolation of genetic material

Answer 1

(vi) Isolation of genetic material 

(iii) Cutting of DNA at specific locations 

(ii) Amplification of gene of interest using PCR Insertion of recombinant DNA into the host cell organism

(i) Obtaining the foreign gene product 

(iv) Down stream processing