Klinefelter’s Syndrome is an aneuploid condition marked by the presence of one or more extra chromosomes in a male (44 + XXY/ 44 + XXXY/ 44 + XXXXY). As we know that, to compensate the dosage of X-linked genes in human male and female, one X-chromosome in females is deactivated and is present as darkly stained body near nuclear envelope. The number of Barr bodies is always one less than the total number of X chromosomes. A human male with Klinefelter’s syndrome, 44 + XXY/ 44 + XXXY/ 44 + XXXXY, can have 1, 2 or 3 Barr bodies, depending on the number of X-chromosome. The presence of extra copy of X-chromosome produces some feminine characters in otherwise male individuals. They are sterile feminised males with enlarged breasts (Gynecomastia).