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Describe in sequence the events that lead to the development of a 3-celled pollen grain from microspore mother cell in angiosperms.

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● Each cell of the sporogenous tissue in a microsporangium acts as a potential pollen mother cell (PMC) or microspore mother cell. 

● PMC undergoes meiotic divisions to form cluster of four cells called microspore tetrad. 

● On maturity, the anther dehydrates and the microspores separate from each other to form pollen grains. 

● The newly differentiated pollen grain has a central nucleus and dense cytoplasm. 

● The protoplast mitotically divides into two unequal cells—bigger vegetative cell which is rich in food reserve and smaller spindle-shaped generative cell with dense cytoplasm and a nucleus. This is called 2-celled stage. 

● In majority angiosperms, pollens are released in this 2-celled stage, whereas in other species, the generative cell divides into 2 male gametes and thus pollen is said to be in 3-celled stage.

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