Flocs are masses of aerobic bacteria associated with fungal filaments to form mesh like structures. These aerobic microbes consume the major part of the organic matter in the effluent. This significantly reduces Biological oxygen demand (BOD) of the effluent.
A small part of the activated sludge is used as inoculum and pumped back to aeration tank. The remaining major part of the sludge is pumped into anaerobic sludge digesters where microbes or bacteria grow anaerobically to produce CH4 or H2S or CO2 or biogas.