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a. What are “molecular scissors”? Give one example.

b. Explain their role in recombinant DNA technology.

OR

Why are molecular scissors so called? Write their use in biotechnology.

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a. The restriction endonucleases are called molecular scissors, as they cut the DNA segments at particular locations, e.g., EcoRI.

b. The restriction enzymes cut the DNA strands a little away from the centre of the palindromic sites, but between the same two bases on the opposite strands. This leaves single stranded portions with overhanging stretches called sticky ends on each strand as they form hydrogen bonds with their complementary cut counterparts. This stickiness at the ends facilitates the action of the enzyme DNA ligase.

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