Question

a. Given below is a single stranded DNA molecule. Frame and label its sense and antisense RNA molecule.

5’ ATGGGGCTC 3’

b. How the RNA molecules made from above DNA strand help in silencing of the specific RNA molecules?

Answer 1

a.

5’ ATGGGGCTC 3’ sense

3’ TACCCCGAG 5’ antisense

5’AUGGGGCUC 3’ sense

3’UACCCCGAG 5’ antisense

b. The two strands of RNA (i.e., sense and antisense) being complementary will bind with each other and form double stranded RNA as a result its translation and protein expression would be inhibited.