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Name the process involved in the production of nematode-resistant tobacco plants, using genetic engineering. Explain the strategy adopted to develop such plants.

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The process involved in the production of nematode-resistant plants is RNA interference or RNAi. Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and antisense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded RNA (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite.

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