In a PCR reaction, following components were taken- ds DNA, Taq polymerase, dNTPs and ds primer, but even after 30 cycles, no amplification of the target DNA could be seen. The reason for this is because the
A. template is DNA and not RNA.
B. ddNTPs are not added in the reaction mixture.
C. primer is double stranded.
D. reverse transcriptase is not added.