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Describe the development of female gametophyte in angiosperms. 

OR 

What is megasporogenesis ? Give an account of development of the female gametophyte. 

OR 

With a neat diagram explain the 7-celled, 8- nucleate nature of the female gametophyte.

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1. Megasporogenesis : 

The process by which . the diploid megaspore mother cell of nucellus undergoes meiosis to form a tetrad of haploid megaspores is known as megasporogenesis. 

2. Development of female gametophyte: 

(i) The diploid megaspore mother cell undergoes meiosis to form a linear tetrad consisting of fourhaploid megaspores. Generally, the chalazal megaspore becomes the functional megaspore. The other three megaspores degenerate. 

(ii) The chalazal megaspore (fertile megaspore) is the first cell of the female gametophyte. It undergoes enlargement and develops into the female gametophyte. The haploid nucellus of chalazal megaspore undergoes three successive free nuclear mitotic divisions to produce eight nuclei. Of these, the first mitotic division results in the formation of two nuclei. 

(iii) Both these nuclei undergo two successive mitotic divisions resulting in the formation of four nuclei at both the poles. In the meantime, one nucleus from each pole called polar nucleus moves towards the centre of the embryo sac and fuse to form a diploid nucleus called secondary nucleus.

(iv) The three nuclei at the micropylar end are organised to form a three-celled structure called egg apparatus, while the other three nuclei at the chalazal end reorganise to form three antipodal cells. The egg apparatus consists of a central cell called egg cell or female gamete which is flanked by two lateral cells called synergids. 

(v) The female gametophyte consists of an egg apparatus, a secondary nucleus and three antipodal cells, A7 celled 8 nucleated structure.

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