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Describe Avery, McCarty and MacLeod’s experiments.

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  • In 1944, Oswald T. Avery, Colin M. MacLeod and Maclyn McCarty proved that the DNA is a genetic material (transforming principle). 
  • They purified DNA, RNA, proteins (enzymes) and other materials from heat killed S-strain cells and mixed them with cells of R-strain bacteria separately to confirm which one could transform living R cells into S cells. 
  • Only DNA was able to transform harmless R-strain into virulent S-strain.
  • They also demonstrated that proteases, RNases did not affect transformation. Thus it was proved that the transforming substance was neither a protein nor-RNA. 
  • When DNA was digested with DNase, there was no transformation. 
  • These experiments proved that the transformation of Live R-strain bacteria into S-strain type was because of DNA of bacteria of S-strain. 
  • Thus, they proved that the DNA is transforming principle.

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