a. A : Explant, B : Callus
b. When auxins are provided to callus in more quantity compared to cytokinins, it gives rise to roots (rhizogenesis) and when cytokinins are provided in more quantity, there is development of shoot (caulogenesis). This induction of organ formation in callus by providing growth hormones in proper proportion is known as organogenesis.
c. Plantlets produced in tissue culture laboratory are transferred to polythene bags containing sterilized soil and are kept on low light and high humidity conditions for suitable period of time. This is known as hardening.
