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How is mature insulin different from proinsulin secreted humans pancreas in human?
Explain how was human functional insulin produced using rDNA technology.
Why is the functional insulim thus produced considered better than the ones used earlier by diabetic patients?

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The proinsulin has an extra stretch called the C peptide chains A and B while the mature insulin has only two polypeptide chains A and B.(b) From the human cell, DNA contaning gene is isolated. The corresponding to A and B, chains of human insulm is introduced plasmide of E . coli to produce insulinA and B . The extracted chain A and B were combined by creating disulphide bonds to form human insulin. (c) The insulin prepared by rDNA technology does not produce senstive allergic reactios and immunological reaction whereas those used earlier produced allergic from reaction and the otjher complication to the forigen protien as earlier , insulin was extracted fi f slaughtered cattle or pigs

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