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How did an American Company, Eli Lily use the knowledge of r-DNA technology to produce human insulin ?

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Two chains of DNA sequence corresponding to A and B chains of human insulin prepared, introduced them into plasmids of E. coli to produce separate A and B chains, A and B chains extracted combined by creating disulphide bonds.

Detailed Answer : 

American Company Eli Lilly used the knowledge of r-DNA technology to produce human insulin in the following way. 

1. Preparation of DNA corresponding to A and B chain separately. 

2. Extraction of plasmid from bacteria. 

3. Insertion of DNA corresponding to A and B chain separately in plasmid. 

4. Transforming the bacteria with this recombinant plasmid. 

5. Expression of the desired product from this DNA . 

6. Purication of A and B chain. 

7. Linking them with disulde bonds so that they act as human insulin.

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