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Differentiate between a template strand and coding strand of DNA.

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Role/Strand Template strand Coding strand
Function Codes for the protein molecule Does not code for anything
Polarity 3' ----> 5' 5' ----> 3'

Detailed answer:

The template strand of double stranded DNA has 3'---> 5'polarity and it transcribes RNA over it in 5' ---> 3' direction whereas its coding strand has 5'----> 3' polarity. It does not transcribe and code for anything. 

It is because RNA polymerases the nucleotides only in 5'-3' direction. The coding strand is also called as the sense strand. It gets displaced during transcription.

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