Between the two amino acids (found on charged tRNA), bound to the two sites of the large sub units of bacterial ribosomes, when two charged tRNAs are brought close enough, peptide bond is formed with the help of ribozyme.
Detailed Answer:
A peptide bond (-CO-NH-) formed between carboxyl group (-COOH) group of amino acid attached to tRNA at P- site and amino group (-NH2) of amino acid attached to tRNA at A-site in presence of enzyme peptidyl transferase, in large subunit of ribosome. The aminoacyl tRNA complex reach the A site and attaches to mRNA codon with the help of its anticodon and then the peptide bonds between amino acids found on charged tRNA.
