The PCR technique is carried out in following to main steps.
(1) Denaturation & Separation of two strands of ds. DNA : The double strand DNA is subjected to denaturation at high temperature (950C) for separation of single stranded strands. These strands now serve as the template strands for synthesis of DNA.
(2) Annealing : The two sets of primers are added which undergo annealing at 3' end of each separated strand.
(3) Extension : The Thermostable DNA (taq polymerase) helps in adding the nucliotides complementary to the template thereby extending the primer. These steps are repeated many times for obtaining several copies of desired DNA.
