Region to be amplified
In PCR each cycle has three steps
(i) Denaturation of DNA sample Unwinding of two strand of DNA of DNA by heating the sample at 92-`94^(@)C`.
(ii) Primer Annealling Primeres get positioned on the exposed nucleotides as per base paring rules.
(iii) Extention of Primers DNA polymerase recognise primeres as start tags and begins to extend the primers using the free nucleotides provided in the reaction and the genomic DNA as template.
With each round of reactions, the DNA doubles.
