Region A Bam HI
Region B Pot 1
Region C amp`.^(R)`
E, coli cloning vector pBR322 showing restriciton sites (Hind III,Eco Rt, Bam HI, Sal 1,Pvu II, Pst I,ClsI), ori and antibiotic resistance genes (amp`.^(R)` and `"tet"^(R)`)
Rop codes for the proteins involved in the replication of the plasmid.