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Illustrate the following with the help of suitable diagrams. a) Binary fission in Amoeba b) Binary fission in Leishmania

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(a) Amoeba is a shapeless tiny unicellular organism that has a porous cell membrane which encloses the cell organelles and cytoplasm. Amoeba reproduces by the common asexual reproduction method called binary fission. After replicating its genetic material through mitotic division, the cell divides into two equal sized daughter cells. The genetic material is also equally partitioned; therefore the daughter cells are genetically identical to each other and the parent cell. In this process, the nucleus of the Amoeba first divides to form two daughter nuclei by the process of Karyokinesis. After the nucleus has divided into two, the process of Cytokinesis takes place in which the cytoplasm in the mother cell divides into two daughter cells. This leads to the formation of the two daughter Amoebae cell having a nucleus and its own cell organelles.

Karyokinesis is the process of the division of the nucleus. It corresponds to the separation of the daughter chromosomes into two daughter nuclei. Karyokinesis is usually followed by Cytokinesis.

Cytokinesis is the process of the division of the cytoplasm. It corresponds to the separation of the daughter nuclei into two daughter cells. Cytokinesis occurs immediately after mitosis.

(b) In Leishmania, binary fission occurs in a definite orientation :
1. Nuclear division is followed by the appearance of a constriction in the cell membrane.
2. The membrane then grows transversely inward from near the middle of the dividing cell.
3. Cytoplasm separates into two equal parts, each with one nucleus.
4. The two daughter cell may separate from each other and behave as two independent organisms.

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