Question

These limitations of the Zuker method and other methods have been largely overcome by using an algorithm originally described by Waterman and Byers (1985) for finding sequence alignments within a certain range of the optimal one by modifications of the trace-back procedure used in dynamic programming.

(a) True

(b) False

I got this question in semester exam.

This question is from MFOLD and the Use of Energy Plots topic in division RNA Structure Prediction of Bioinformatics

Answer 1

Right option is (a) True

For explanation I would say: This method efficiently calculates a large number of alternative structures, up to a very large number, within a given energy range of the minimum free-energy structure. The method has been used to demonstrate that natural tRNA sequences can form many alternative structures that are close to the minimum free-energy structure and that base modification plays a major role in this energetic stability.