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Recombinant plasmids are added to a bacterial culture that has been pretreated with _________________ ions.

(a) iodine

(b) magnesium

(c) calcium

(d) ferric

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Right choice is (c) calcium

The explanation: Recombinant plasmids are added to a bacterial cell culture that is competent for taking up the plasmids from the medium. A culture can be made competent by treatment with calcium ions.

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