Question

Greater accuracy in the determination of the position of a particle with an optical microscope can be had, if the beam of light used 

(a) has higher wavelength 

(b) is polarised 

(c) has higher frequency 

(d) has greater intensity.

Answer 1

The correct option is (c) has higher frequency.

Explanation:

The resolving power of a microscope is given by

R = 2μ sin θ/1.22 λ Where μ = the refractive index of the medium between the object and the objective

 θ = angle subtended by the radius of the objective on any one object 

λ = wavelength of light used

for greater accuracy, wavelength of light used should be smaller, or conversely, the frequency of the light should be higher.