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The best and safest method of sterilization is:
1. Boiling for 30 minutes
2. Flaming
3. Autoclaving
4. Immersion in chemical germicide

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Correct Answer - Option 1 : Boiling for 30 minutes

Explanation

  • It is a technique of removing or destroying all viable forms of microorganisms.

 Boiling Method.

  • It is a method of moist heat sterilization at 100 degrees Celcius.
  • It is a simple type of water disinfection.
  • Boiling water at a high temperature (100 degrees C) is capable of destroying almost all forms of bacteria and viruses.
  •  The time duration should be at least 20 minutes.

Physical Methods of Sterilization

  • Heat sterilization
    • Moist heat
    • Dry heat
  • Filtration
  • Irradiation
  • Sonic waves
  • Pascalization
  • Solar method

Chemical Sterilization

  • Gases
    • Ethylene oxide
    • Formaldehyde
    • Nitrogen dioxide
    • Ozone
  • Liquid 
    • Hydrogen peroxide
    • Glutaraldehyde
    • Hypochlorite

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