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What is the full name of "ELISA"?
1. Enzyme Licensed Immune Solution Activity
2. Enzyme linked immune sorbent assay
3. Enzaam Linked Integrated Solution Activity
4. Enzyme lyases immune solution assay

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Correct Answer - Option 2 : Enzyme linked immune sorbent assay

Concept-

  • ELISA means Enzyme-Linked Immuno-sorbent Assay.
  • ELISA techniques serve the purpose of early diagnosis.
  • ELISA is based on the principle of antigen-antibody interaction.
  • Infection by a pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesized against the pathogen.

Explanation-

  • ELISA uses an enzyme to detect the binding of antigens and antibodies.
  • The enzyme converts a colorless substrate to a colored product, indicating the presence of Ag: Ab binding.
  • An ELISA can be used to detect either the presence of Antigens or antibodies in a sample depending on how the test is designed.
  • ELISA was developed in 1970 and became rapidly accepted.
  • ELISA is used for the diagnosis of AIDS.
  • The working principle of ELISA is antigen-antibody interaction.

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