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Which of the following pairs is correctly matched ?


1. Ligases - Molecular Scissors
2. Ligases - Molecular Stitchers
3. Restriction Endonucleases - Molecular Stitchers
4. DNA Polymerase - Molecular Scissors

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Correct Answer - Option 2 : Ligases - Molecular Stitchers

The correct answer is Ligases - Molecular Stitchers.

  • Ligase is like a molecular stitcher, they are ubiquitous and essential for all cells and perhaps at all times.
  • DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two single-stranded DNA fragments by catalyzing the formation of an inter-nucleotide ester bond between phosphate and Deoxyribose.
    • It is active during DNA replication, DNA repair, and DNA recombination.
  • There are two forms of DNA ligase: one requires ATP and the other NAD. NAD (+) dependent DNA ligases are present in bacteria, some Entomopox viruses, and Mimivirus while ATP-dependent DNA ligases are ubiquitous.

  • Restriction endonucleases are the restriction enzymes that cleave the DNA at specific sites so they are known as molecular scissors.
  • DNA polymerases are enzymes that play a key role in DNA replication.
    • DNA replication is the process of splitting an existing double-stranded DNA molecule into two single strands of DNA.

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