(i) BAC and YAC
BAC- Insert size-50-500 kb
YAC-Insert size-250-1000 kb
(ii) pBR322 and pUC 19-
pBR322- contains two antibiotic resistance genes ,
pUC 19- contains Lac Z gene
(iii) M-13 and lambda phage-
M-13- circular single stranded, lambda
phage- linear double stranded
(iv) Cosmid and Plasmid
Cosmid - having features of plasmid and cos sites of phage lambda, insert size 30- 40Kb
Plasmid- small, circular, extra-chromosomal, self -replicating, naturally present in bacteria insert size 0.5-8 Kb
(v) Transformation and Transfection
Transformation- Cold CaCl2 treated competent bacterial cells to introduce Rdna
Transfection - Transfer rDNA in to host cells by mixing foreign DNA with charged substances like calcium liposomes/ calcium phosphate/ DEAE dextran
