Rajesh went to a doctor to check his blood glucose level. Doctor used a reagent which is colourless and turns pink in presence of glucose. More the concentration of glucose, greater the intensity of colour. This colour intensity can be quantitated using an instrument ‘colorimeter’. Following table gives colorimeter readings for four standard glucose concentrations.
Note that all colorimeter readings values above 0.05 are considered positive.
Rajesh’s blood sample showed a reading 0.75.
A standard graph of OD values against the concentration of glucose is given.
(A) What is the molar concentration of glucose in Rajesh’s blood? _________ Show extrapolation in the graph and calculations in the box
Doctor than gave Rajesh 100 g glucose to eat and tested his urine and blood samples at the intervals of 30, 60, 90 and 120 min. The readings in the colorimeter were as follows:
Min |
Reading for blood Sample |
Reading for urine sample |
30 |
0.55 |
0.33 |
60 |
0.75 |
0.15 |
90 |
1.0 |
0.25 |
120 |
1.2 |
0.35 |
(B) Plot graphs of glucose concentration in blood and urine against time in the given graph paper in the answer sheet.
(C) What is the concentration of glucose(in mg%) in the blood reaching nephron at 80 min?
(D) What is the concentration of glucose above which the kidneys start removing it in urine?