In this method, hybridization of different species could produce variants of economic value as follows:
- Isolation of desired single cells.
- Digestion of cell wall by pectinase & cellulase enzyme for exposure of protoplast.
- Fusion of protoplast by Polyethylene glycol(PEG).
- Hybrid protoplast culture resulting in desired variety of plant eg., Pomato is obtained by somatic hybrid of potato and tomato.