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Highlight the salient features that are required to facilitate cloning into a vector.

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Highlight the salient features that are required to facilitate cloning into a vector. 

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Salient features of a DNA cloning Vectors: 

  • Size: small enough to be easily separated from the chromosomal DNA of the host bacteria. 
  • Ori site; must have the site for DNA replication that allows the plasmid to replicate separately from the host cell‘s chromosome. 
  • Multiple Cloning sites: a stretch of DNA with recognition sequence for many different common restriction enzymes. 
  • Selectable marker genes 
  • RNA polymerase promoter sequence
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