Question

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA? 

(a)  Sal I 

(b)  Pvu I 

(c)  EcoRI 

(d)  Hind III 

Answer 1

The correct option is (a) 

• Sal I is a restriction endonuclease used for molecular biology methods to cleave DNA at the recognition sequence 5′-G/TCGAC-3′, generating fragments with 5′-cohesive ends.

Answer 2

Correct option   (a)  Sal I