Question

Summarize the process by which the sequence of DNA bases in Human Genome Project was determined using the method developed by Frederick Sanger. Name a free living non-pathogenic nematode whose DNA has been completely sequenced.

Answer 1

• The sequences were arranged based on some overlapping regions present in them (Alignment of these sequences was not humanly possible)

• Therefore, specialized computer based programme was developed.

• These sequences were subsequently annotated and were assigned to each chromosome-

• Chromosome  1

• Caenorhabditis elegans