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What are the conditions necessary for fixation of atmospheric nitrogen by Rhizobium. What is their role in N2 -fixation?

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Rhizobium bacteria need symbiotic association with legume plants to carry out nitrogen fixation. Root nodules contain the necessary enzymes for nitrogen fixation and thus enable rhizobium to fix nitrogen. The enzyme nitrogenase facilitates the conversion of nitrogen into ammonia which is the first stable product of nitrogen fixation. Ammonia is then converted into glutamic acid. Glutamic acid is then utilised by plants to make amino acids; which are then utilised to make protein. 

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