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 The calculations for respiratory balance sheet are based on some assumptions which are as follows:

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There is a sequential and orderly pathway in which one substrate makes the next substrate. Glycolysis, TCA cycle and ETS pathway follow one after another. 

• NADH is synthesized in glycolysis and is transferred into the mitochondria. The NADH undergoes oxidative phosphorylation within the mitochondria. 

• None of the intermediates in the pathway are utilised to synthesise any other compound. 

• Glucose is the only substrate undergoing respiration. No other alternative substrates are entering in the pathway at any stage. 

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