There is a sequential and orderly pathway in which one substrate makes the next substrate. Glycolysis, TCA cycle and ETS pathway follow one after another.
• NADH is synthesized in glycolysis and is transferred into the mitochondria. The NADH undergoes oxidative phosphorylation within the mitochondria.
• None of the intermediates in the pathway are utilised to synthesise any other compound.
• Glucose is the only substrate undergoing respiration. No other alternative substrates are entering in the pathway at any stage.
