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What are the assumptions made during the calculation of net gain of ATP?

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  • It is assumed that various parts of aerobic respiration such as glycolysis, TCA cycle and ETS occur in a sequential and orderly pathway. 
  • NADH produced during the process of glycolysis enters into mitochondria to undergo oxidative phosphorylation.
  • Glucose molecule is assumed to be the only substrate while it is assumed that no other molecule enters the pathway at intermediate stages.
  • The intermediates produced during respiration are not utilized in any other process.

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