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Define totipotency of a cell. List the requirements of the objective to produce somaclones of a tomato plant in commercial scale.

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Totipotency is defined as the capacity of any plant cell to generate a whole plant. 

Requirements are

  • An explant, i.e. any part/tissues of a tomato plant
  • Nutrient medium containg a carbon source like sucrose, inorganic salts, aminoacids, vitamins and growth regulators like auxins and cytokinins.
  • Sterile conditions.

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