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Comment on PBR 322 plasmid.

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PBR 322 plasmid is the most commonly used plasmid vector. In this plasmid, two marker sites TetR (Tetracycline resistant) and AmpR (Ampicillin resistant) are found. It contains recognition sites for 12 different restriction enzymes. Desired DNA (foreign DNA) is inserted in between TetR and AmpR gene with the help of restriction enzyme.

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