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Write a short note on the importance of Bacteriophage as a clonal vector

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Bacteriophages are a better vector than the plasmids due to the following reasons: 

  • It can clone DNA segment of relatively large size (24 kbp). 
  • Every bacteriophage produces one plaque area in the culture through which testing is comparatively easy. 

λ (Lambda) phage is more widely used as a vector than M13 phage because λ phage is having linear and double-stranded DNA and it is phage of E.coli and large-sized foreign DNA can be easily inserted in it’s DNA.

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