0 votes
944 views
in Genetic Engineering by (47.6k points)
closed by

Describe different blotting techniques in details

1 Answer

+1 vote
by (48.2k points)
selected by
 
Best answer

Blotting Technique: In this technique, DNA segments are derived on agarose gel by electrophoresis and are then transferred and stabilised on a nitrocellulose filter. These are then identified by hybridization with DNA probes. This process is called blotting technique. 

1. Southern Blotting Technique: E.M. Southern (1975) first used this method and transferred DNA segments on the nitrocellulose filter. This technique was named a Southern blotting technique. By this technical analysis of DNA, segments are done.

2. Northern Blotting Technique: Alwin et al (1979) transferred RNA segments after electrophoresis on amino benzyl oximetry (OBM) membrane in place of nitrocellulose filter. This technique was named as Northern blotting technique. This technique is used for the analysis of RNA segments. 

3. Western Blotting Technique: Tobin et al. (1979) first break down proteins into polypeptides with the help of sodium-do-decyl sulphate (SDS). After separating them with the help of electrophoresis, transferred these on nitrocellulose filter or nylon membrane. These were then decoded on an X-ray plate and identified the protein. By this technical analysis of proteins is done. This is called western blotting technique.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.

Categories

...