1. Culture medium: The nutrient medium, the chemical composition of which is precisely known and which is used to raise tissue culture and its multiplication is known as culture medium.
2. Protoplast: The contents of the plant cell devoid by the cell wall collectively constitute the protoplast. Protoplast is thus a plant cell without a cell wall.
3. Explant: The part of the plant used for raising tissue-culture is known as explant. Meristematic tissue, part of root, stem or leaves and flower and floral parts can be used as explant.
4. Transgenic plant: Genetically modified plants are known as transgenic plants. Transgenic plants are developed by introducing desired features through recombinant DNA technique through genetic engineering
