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What are the different components of an ideal culture medium?

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An ideal culture medium should contain all the mineral nutrients required for plant growth. The most commonly used culture medium is M.S. medium proposed by Murashige and Skoog (1962). The basic components of an ideal culture medium are as follows: Basic Composition of culture medium. 

1. Vitamins: B1, B3, B5 and B6 

2. Amino acid-Glycine (Simplest) 

3. Complex Nutrients 

  • Casein hydrolysate
  • Coconut milk
  • Malt extract
  • Tomato juice
  • Yeast extract
  • Corn milk 

4. Phyto hormones – Such as 

5. Auxins – Root Differentiation

  • Indole butyric acid
  • Naphthalene acetic acid
  • Napthoxy acetic acid

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