Use app×
Join Bloom Tuition
One on One Online Tuition
JEE MAIN 2025 Foundation Course
NEET 2025 Foundation Course
CLASS 12 FOUNDATION COURSE
CLASS 10 FOUNDATION COURSE
CLASS 9 FOUNDATION COURSE
CLASS 8 FOUNDATION COURSE
0 votes
665 views
in Bio-Medical Technologies by (45.7k points)
closed by
What is RIA?

1 Answer

0 votes
by (46.2k points)
selected by
 
Best answer

Radio Immuno Assay (RIA):

  • Radioimmunoassay is an analytic technique which is used from the last many years.
  • Here, the molecule which is to be analysed, which acts as an antigen is marked with a radiolabelled substance.
  • The labelled and normal antigen molecules are allowed to react with a specific antibody.
  • A comparative analysis of the reaction is done. In this technique, the radioisotope is used as a marker molecule.
  • This technique was invented by Rosalyn and Yalow. Now – a – days it has become an important diagnostic technique.
  • This is especially important for the analysis of those biochemical factors that are present in very minute concentration (microgram, nanogram or picogram) and cannot be analysed by the traditional gravimetric and volumetric processes.
  • The radioisotopes used in radioimmunoassay are high specificity molecules, which provide very high sensitivity to this technique.
  • In this technique, the standard solution of different concentration of normal molecules of the substance to be analysed is used with the solutions of the radiolabelled substance of similar concentrations.
  • This mixture is allowed to react with antibodies. At the stage of equilibrium, antigen-antibody complexes are absorbed by the suitable reagent.
  • The precipitated and supernatant parts are separated and by measuring the radioactivity, the concentration of the substance is estimated.
  • A major characteristic of Radio Immuno Analysis is that the patient does not suffer from any side effect.
  • He is never treated with the radioisotopic molecule because the whole process is executed outside the body.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.

Categories

...