Use app×
Join Bloom Tuition
One on One Online Tuition
JEE MAIN 2025 Foundation Course
NEET 2025 Foundation Course
CLASS 12 FOUNDATION COURSE
CLASS 10 FOUNDATION COURSE
CLASS 9 FOUNDATION COURSE
CLASS 8 FOUNDATION COURSE
0 votes
767 views
in Biology by (46.2k points)
closed by

(i) What are restriction endonucleases? Give the rules of their nomenclature.

(ii) Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.

1 Answer

+1 vote
by (49.5k points)
selected by
 
Best answer

(i) They cut DNA duplex at specific points. 

They are molecular scissors. 

Rules : Restriction endonucleases are named by using first letter of the name of genus followed by first two letters of species from which these enzymes are isólated. All the three letters are written in italics.

(ii) Each restriction endonuclease recognises a specific palindromic base sequence in foreign DNA and cuts sugar phosphate backbone of both strands of double helix at specific points. These ends are called as sticky ends. The sticky ends of 2 different molecules of DNA cut by same restriction endonuclease arc thenjoined by ligase to form a recombinant DNA.

Welcome to Sarthaks eConnect: A unique platform where students can interact with teachers/experts/students to get solutions to their queries. Students (upto class 10+2) preparing for All Government Exams, CBSE Board Exam, ICSE Board Exam, State Board Exam, JEE (Mains+Advance) and NEET can ask questions from any subject and get quick answers by subject teachers/ experts/mentors/students.

Categories

...