Chargaff’s rule of equivalence : Deoxy ribonucleic Acid (DNA), the genetic material is made up of four types of organic nitrogenous bases : adenine (A), guanine (G), thymine (T) and cytosine (C). Of these, A and G are the purines and T and C are the pyrimidines. Chargaff gave the base pairing rule or the rule of base equivalence which states that only one purine can combine with one pyrimidine. That means A can combine with T and G with C. Two purines or two pyrimidines cannot combine with each other; if they do so, there w ill be a sudden change in the characteristic of an organism. This sudden change is called mutation.