Site – Directed Mutagenesis: In nature, mutations arise spontaneously but they are rare such as sickle cell anemia. The most powerful method of introducing point mutation into a gene is known as Oligonucleotide or site-directed mutagenesis.
Change in a single nucleotide base pair is called “point mutation”. Site-directed mutagenesis is a molecular biology technique in which a mutation is created at a specific site in the DNA molecule. Using this technique specific point of a gene can be mutated. Therefore, this method has been used to understand the function of many genes. Moreover, this technique can only be used when nucleotide sequence of gene is known.
