(i) PCR:
• Amplification of desired segment of DNA within hours.
• No host cell required.
Gene cloning:
• Amplification of a desirable gene takes more time under lab condition.
• Host cell is required.
(ii) Batch culture:
• In this culture, the same medium and all the cells produced are retained in the culture vessel. Fresh medium is not added.
• The cell number of biomass exhibits a typical sigmoid curve.
Continuous culture:
• In this culture, both cells and used medium are taken out from the continuous culture and replaced by equal volume of fresh medium.
• Here, the cell population is a maintained in a steady state by regularly replacing a portion of used or spent medium.
