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The diagram, below represents an experiment conducted to prove the importance of a factor in Photosynthesis. Study the same and then answer the questions that follow :

(i) Name the factor being studied in this experiment ? 

(ii) Why was the plant kept in a dark room before conducting the experiment ? 

(iii) Why was the experimental leaf then kept in 

(1) boiling water (2) methylated spirit ? 

(iv) Name the solution used to test for presence of starch in the leaf. 

(v) What will we observe in the experimental leaf at the end of the starch test ? 

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(i) Sunlight 

(ii) Plant is kept in a dark room before conducting the experiment, to destarch it. 

(iii) 1. In the boiling water, it was kept to kill the cells. 2. In the methylated spirit, it was kept to dissolve the chlorophyll. 

(iv) Iodine is used to test for presence of starch in the leaf. 

(v) On treating the leaf with iodine solution it shows that the portion of leaf covered with the strip of black paper does not give positive test for starch. The covered portion of leaf indicates the absence of starch after iodine test because it couldnot get light to perform photosynthesis. Hence it is proved that in the absence of light the leaf can not manufacture the starch. 

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